Equipment
New microscope setups and imaging techniques developed in response to feedback from users of NIC@IMCB resulting benefit for Biopolis and regional research community.
Made possible with several corporate contributors including Chroma Technology, microLAMDA Pte. Ltd., Tokai Hit, Okolab, Prior Scientific, Newport, Einst, Teledyne Photometrix, Oxford Instruments, Laser 21 and Coherent Inc.
A1RHD25 Confocal Microscope System
1) A1RHD25 Confocal
Featuring industry leading 25 mm large Field Of View coupled with hybrid Galvano Resonant
scanner. Combined with Nikon's Ti2-E motorized inverted microscope this system provides versatile
imaging modalities. An on stage incubator enables live cell imaging.
Imaging Modalities
- Confocal (4 laser lines: 405/488/561/640)
- Widefield Fluorescence
- Live Cell Imaging
2) ECLIPSE Ti2-E / DS-Qi2
Featuring a fully motorized inverted microscope coupled with high sensitivity DS-Qi2 14 bit
monochrome camera. A 16.25 megapixel CMOS chip sensor that high pixel density, high sensitivity
and low noise. DS-Qi2 delivers exceptional image quality with unparalleled detail and clarity.
Imaging Modalities
- Widefield Fluorescence
- DIC
- Brightfield
System Configuration:
- Confocal Microscope System A1RHD25
- Inverted Microscope ECLIPSE Ti2-E
- Monochrome Microscope Camera DS-Qi2
- Imaging Software NIS-Elements C
ECLIPSE Ti2-E Inverted Fluorescence Imaging System
The ECLIPSE Ti2-E is an advanced research inverted microscopy system that offers unparalleled advanced widefield fluorescence imaging capabilities. This microscope is designed to provide exceptional imaging speed and accuracy, thanks to its range of motorized components that allow for precise and repeatable positioning of the sample and imaging components.
With its widefield fluorescence imaging capabilities, the ECLIPSE Ti2-E enables the visualization of fluorescently labeled structures and molecules with incredible resolution and sensitivity. Its cutting edge technology makes it the perfect tool for researchers and scientists who demand the highest quality imaging results.
Imaging Modalities
- Widefield Fluorescence
- DIC
- Brightfield
System Configuration:
- Inverted Microscope ECLIPSE Ti2-E
- Scientific CMOS Camera Andor Zyla
- Fluorescence LED Illumination System D-LED
- Imaging Software NIS-Elements AR
N-STORM/TIRF + Live Cell Imaging System
Nikon STochastic Optical Reconstruction Microscopy (N-STORM) allows super resolution imaging of up to 20x improvement over conventional microscopy imaging modalities. This system also serves as the default Widefield Live Cell Imaging station.
Imaging Modalities
- STORM
- Widefield Fluorescence
- DIC
- Brightfield
- Live Cell Imaging
System Configuration:
- Super Resolution Microscope System N-STORM
- Inverted Microscope ECLIPSE Ti-E
- EMCCD Camera Andor iXon Ultra 897
- EMCCD Camera Photometrics CoolSNAP HQ2
- Stage Top Incubator System Tokai Hit STXG-WSKMX
- Imaging Software NIS-Elements AR
ECLIPSE Ni-E/DS-Ri2 Upright Brightfield System
The Eclipse Ni-E is a fully motorized model designed to provide high-quality imaging across a wide range of applications. The microscope equipped with a DS-10 camera, FX format CMOS sensor, capable of capturing high resolution 6K and switching between color and monochrome. This system is able to perform brightfield, darkfield, phase contrast, DIC, and fluorescence imaging.
With its fully motorized design, this system is easy to use for multidimensional image acquisition as well as large image stitching.
Imaging Modalities
- Widefield Fluorescence
- DIC
- Phase Contrast
- Darkfield
- Brightfield
System Configuration:
- Upright Microscope ECLIPSE Ni-E
- Dual Mode Microscope Camera DS-10
- Epi-fluorescence Illuminator INTENSILIGHT
- Imaging Software NIS-Elements AR
ECLIPSE Ts2FL Routine Inverted System
ECLIPSE Ts2 inverted microscope is a compact and easy to use cell culture microscope. It equipped with Nikon’s industry-leading CFI60 optical system and LED illumination for clear diascopic and epifluorescence imaging.
The ECLIPSE Ts2 is also equipped with the colour DS-Fi3 C-mount camera, delivering superior color reproduction, and high quantum efficiency for various observations methods such as brightfield, phase contrast, Emboss Contrast, and fluorescence.
Imaging Modalities
- Widefield Fluorescence
- Phase Contrast
- Brightfield
System Configuration:
- Inverted Routine Microscope ECLIPSE Ts2FL
- Monochrome Microscope Camera DS-Fi3
- Microscope Camera Control Unit DS-L4
SMZ18 Stereo Microscope System
The SMZ18 is a powerful stereo microscope that boasts a remarkable 18:1 zoom ratio and advanced stereo microscope optics. It delivers crystal clear and intricate views of biological specimens. With state-of-the-art LED DIA Base and OCC illuminator, the SMZ18 generates minimal heat while providing long-lasting illumination, which enhances the contrast of uneven surfaces.
Moreover, the microscope comes equipped with the DS-Fi3 camera – a compact and high-performance C-mount camera that delivers superior color reproduction and high quantum efficiency, making it ideal for brightfield and fluorescence observations.
Imaging Modalities
- Widefield Fluorescence
- Brightfield
System Configuration:
- Research Stereo Microscope SMZ18
- Monochrome Microscope Camera DS-Fi3
- Imaging Software NIS-Elements AR
NIS-Elements AR Offline Analysis Software
NIS-Elements is a highly intuitive software that provides an extensive array of features and capabilities that aid researchers in analyzing and processing microscopy images.
With its powerful analysis function, researchers can easily identify and measure various features, including cell size, shape, and distribution, as well as intensity and time measurement.
Additionally, the software’s EDF (extended depth of focus), deconvolution, and 3D volume rendering functions allow users to gain valuable insights into their experimental results.
System Configuration:
- Imaging Software NIS-Elements AR