A1R+si Confocal

Microscope setup (with incubator) for Confocal 3D Optical Sectioning

Capturing high quality images of cells and molecular events at high speed

  • Galvano scanner for high resolutions (16 Megapixels) and high speed (10fps) scanning
  • Ultrafast (420fps) scanning with resonant scanner
  • Stimulation + Imaging with hybrid scanner

Multicolour Imaging, Spectral Imaging, Simultaneous photoactivation and imaging, photoactivation dye, FRAP, FRET, Caged Compound, Calcium imaging dye

Multicolour Imaging  
  • 4 Colour Fluorescence + DIC
  • Simultaneuos/Custom Sequential excitation

< Zebrafish labeled with 4 probes. (From A1+ Brochure)
Nucleus (Blue): Hoechst33342, Pupil (Green): GFP,
Nerve (Y): Alexa555, Muscle (Red): Alexa647.

Spectral Imaging  
  • One shot 32 channels
  • Seperate overlapping fluorescence spectra
  • Elimintating autofluorescence
<Spectral and unmixed image of 5 probes labeled HeLa cells (From A1+ Brochure)
<Unmixing result.
From left: Nucleus(DAPI), Vinculin(Alexa488), Vimentin(Alexa568), Tubulin(Alexa594), Actin (Alexa633)
N-STORM/TIRF + Live Cell

Microscope setup for Super-resolution N-STORM, TIRF and Live Cell Imaging

  • N-STORM: 10X better resolution than conventional microscopy
  • TIRF: Study of cell event close to plasma membrane
  • Live Cell: Equipped with on-stage incubator, shutter and filter wheel for live cell imaging
N-STORM (STochastic Optical Reconstruction Microscope)
  • Super-resolution image with localization of each fluorophore
  • Multichannel 2D/3D (3 colours)
  • Resolution XY:~ 20nm; Z: ~50nm
  • N-STORM dyes recommended
  • Continuous mode available
  • Contact us for sample preparation protocol and enquiries
2 Colours N-STORM :
Red-Microtubules (Cy3-Alexa647)
  Green- Mitochondria (Alexa405-Alexa647)
TIRF (Total Internal Reflection Fluorescence)
  • Excites fluorophores within 100nm above coverslip
  • Suitable for observation events occur close to plasma membrane
  • 4 Colours TIRF
Cells immunocytochemically labeled for the protein tubulin under widefield and TIRF observation
(Courtesy of MicroscopyU.com)
Ni-E: Upright Microscope

Microscope for slide viewing: Brightfield, Darkfield, Phase contrast, DIC and Fluorescence

Large image stitching of HE staining slide under 10X objective
Brightfield and Darkfield observation of mouse blood (40X objective)
AZ100: Multizoom Microscope
Configuration: Brightfield, DIC and Fluorescence
Magnification: 5X-400X
Extended Depth of Focus (EDF) image can be constructed with NIS-Elements
Sample view under 5X objective with different zooms
SMZ18: Stereomicroscope
Configuration: Brightfield. Oblique Coherent Contrast (OCC) and Fluorescence
Magnification: 7.5X to 135X
Extended Depth of Focus (EDF) image can be constructed with NIS-Elements
EDF image of water plant constructed with Z-stack image
TS100: Cell Culture Microscope
Nikon TS-100-F
Nikon Camera controller DS-L3 + Fi1c
Configuration: Brightfield, Phase Contrast, Fluorescence (FITC and TRITC)
Biostation IM

Biostation IM


Compact unit for long-term, time-lapse, live-cell imaging with

  • Microscope
  • Incubator
  • High Sensitivity Camera

Configuration: Phase contrast + 2 Fluorescence

Application: Wound healing, Cell migration etc

Imaging System: DS-L3

Nikon Camera controller DS-L3

  • Camera Controller
  • Simple measurement
    Distance between 2 points, length of perpendicular line, angle, diameter and circumference of circle, area of polygon etc.
  • Touch Panel

* Please prepare thumbdrive as storing media

Imaging System: NIS-Elements

Nikon Software: NIS-Elements

  • Imaging software controls motorized microscope components, cameras and lasers
  • 6D imaging capability

* Users should provide their own media to export generated data.


Image processing and analysis:

  • Manual measurement
  • Auto-measurement (binarization, object count)
  • Time measurement
  • Intensity measurement
  • HDR image capture
  • EDF
  • Deconvolution
  • Bio-analysis
  • 3D video rendering etc